Figure 1 From: TBP2 is a substitute for TBP in Xenopus oocyte transcription TBP2 is actively down regulated upon meiotic maturation. (A) Western blot analysis of TBP expression in oocytes, eggs and late blastula (stage 9) embryos with four different antibodies; 58C9, SL27, SL30 and SL33. Asterisk indicates non-specific bands. (B) Western blot analysis of TBP2 expression in oocyte germinal vesicles and embryonic nuclei with a TBP2-specific monoclonal antibody 3E6. (C) Stage VI Xenopus oocytes were incubated in MBSH buffer containing 1 μg/ml progesterone. Batches of 15 oocytes were collected at indicated time intervals and analyzed by western blotting. (D) Stage VI Xenopus oocytes injected with TBP and HA-TBP2 mRNA were incubated in MBSH buffer containing 1 μg/ml progesterone. Batches of 15 oocytes were collected at indicated time intervals and analyzed by western blotting. (E) Oocytes expressing exogenous TBP were treated with either progesterone (1 μg/ml) or cycloheximide (15 μg/ml). Batches of 15 oocytes were collected 4 hr and 16 hr after the treatment and analyzed by western blotting. Asterisk indicates non-specific bands.Back to article page