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Table 1 Oligonucleotides used in this study

From: Light-dependent roles of the G-protein α subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei)

Name

Sequence

Application

cbh1SF

5'-TCGGCCTGCACTCTCCAATCC-3'

PCR fragments used as probes for northern blots

cbh1SR

5'-TGGAGTCCAGCCACAGCATG-3'

 

18SRF

5'-GGTGGAGTGATTTGTCTG-3'

 

18SRR

5'-CTTACTAGGGATTCCTCG-3'

 

hfb1F

5'-CTGAACACCTCCAGTCAAC-3'

 

hfb1R

5'-TATTGGAGAATAAGAATGGC-3'

 

hfb2F

5'-TCAAGATGCAGTTCTTCG-3'

 

hfb2R

5'-CATTGCTTTAGAAGGTGC-3'

 

gna1aa5F

5'-ACACGAACGCCCAAATCTG-3'

Construction of vector pBgna1Q204L

gna1aa5R

5'-CTTTCGCTCAGATCGCTG-3'

 

gna1aa3F

5'-GCCAGCGATCTGAGCGAAAGAAG-3'

 

gna1aa3R

5'-GGGCGACGGCACATTAACATAG-3'

 

gna1aa5NF

5'-atgtcgacCAAATCTGCGTCTTCCACAC-3'

 

gna1aa3NR

5'-atgggcccCGGCACATTAACATAGCTTG-3'

 

gna1F

5'-TAAGCGAAGGTTCTCCTG-3'

cDNA amplification

gna1R

5'-ATTGGCATTTCATTCCTC-3'

 

gna1D5F

5'-atgggcccCATCTCCACAGTTTCCAGG-3'

Construction of vector pDELgna1

gna1D5R

5'-atgtcgacTGTTGGCGACTGTGAATG-3'

 

gna1D3F

5'-atggatccTTAGACGACTTGTTGATTGTTG-3'

 

gna1D3R

5'-attctagaCCCAATGCTTATAGAACATC-3'

 
  1. Mutated bases are given in bold, artificially introduced restriction sites are given in lower case letters.
  2. PCR = polymerase chain reaction.