Figure 6

SMAD phosphorylation. (a) A concentration-dependent phosphorylation of SMAD-1 mediated by bone morphogenic protein (BMP)2 is shown at the indicated timepoints in C2C12 cells by western blotting. Analysis of SMAD-1 and actin levels acts as loading control. (b) Time-dependent phosphorylation of SMAD-1 by BMP2 at a concentration of 0.3 nM. (c) Diagram of the time-dependent phosphorylation levels of SMAD-1 without ligand or induced by BMP2 at the indicated concentrations. The data were obtained by scans of western blot exposures. pSMAD signals were quantified and normalized to total SMAD-1 levels using the software ImageJ. (d) Concentration-dependent phosphorylation of SMAD-1 mediated by heterodimeric core BMP wild type (coreBMP2wt)/L51P.