Skip to main content

Table 3 Primers used for PCR amplification and sequencing.

From: Evolution and diversity of Rickettsiabacteria

Gene

Description

Primer name

Primer sequence (5'-3')

Reference

16S

16S rDNA

27f

AGAGTTTGATCCTGGCTCAG

[81]

  

rssur

GAAAGCATCTCTGCGATCCG

[15]

atpA

ATP synthase F1 alpha subunit

atpAf2

ATCAAGCGTTGCACAGATAG

this study

  

Vitr

CGACTTACCGAAATACCGAC

[33]

  

atpA536r

GGAAGTGCCGTAAGTGAACC

this study

gltA

citrate synthase

rcit133f

GGTTTTATGTCTACTGCTTCKTG

[10]

  

rcit1197r

CATTTCTTTCCATTGTGCCATC

[10]

coxA

subunit I of cytochrome C oxidase

coxAf2

ACAGCCGTTGATATGGCTA

this study

  

coxA1413r

CATATTCCAACCGGCAAAAG

this study

  

coxA322f

GGTGCTCCTGATATGGCATT

this study

  

coxAr1

CATATTCCAGCCGGCAAAAG

this study

  1. For AtpA, the primers used in tandem are atpAf2 as a forward primer and either VitR or atpA536r as reverse primers. For amplification of coxA, the primers used are coxAF2 with coxA1413r or coxA322f with coxAr1.