Table 1 Primers used in the study.
From: Genetic determinants of mate recognition in Brachionus manjavacas(Rotifera)
Gene | GenBank Accession Number | Primer | Primer sequence (5'-3') |
|---|---|---|---|
MMR-A4 | GQ374516 | MMRA4F | ATGAAATCAATTTTATGTTCCTSCTG |
| Â | Â | MMRA4R | TTAATCARAATAAAGAGGAAAAG |
MMR-A7 | GQ374517 | MMRA7F | ATGAAATCAATTTTATGTATCCTSCTG |
| Â | Â | MMRA7R | GTATTTTTTATTTTTGATAAAAATCTG |
MMR-B3 | GQ374518 | MMRBF | GTACCAGTYAAGCAAATAGCTGAACC |
| Â | Â | MMRBR | ATATTTTAAATTTAACCTTGAACC |
MMR-B5 | NA | MMRBF | GTACCAGTYAAGCAAATAGCTGAACC |
| Â | Â | MMRBR | ATATTTTAAATTTAACCTTGAACC |
MMR for RNAi (vector-specific) | NA | RNAiF | ACCATGATTACGCCAAGCTCAG |
| Â | Â | RNAiR | GTTTTCCCAGTCACGACGTTG |
MMR (vector-specific for double-stranded RNA synthesis) | NA | RNAi-T7F | TAATACGACTCACTATAGGACCATGATTACGCCAAGCTCAG |
| Â | Â | RNAi-T7R | TAATACGACTCACTATAGGGTTTTCCCAGTCACGACGTTG |
GDP-mannose 4,6-dehydratase (MAN) | FJ829249 | MANF MANR | GGGGTATGTTTTGTCCCAATC ACCCAGCAGCATATGGTTTC |
| Â | Â | MANF-T7F MANR-T7R | TAATACGACTCACTATAGGGGGGTATGTTTTGTCCCAATC TAATACGACTCACTATAGGACCCAGCAGCATATGGTTTC |