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Figure 5 | BMC Biology

Figure 5

From: Genes encoding novel secreted and transmembrane proteins are temporally and spatially regulated during Drosophila melanogasterembryogenesis

Figure 5

Subcellular localization of uncharacterized proteins. S2R+ cells were transiently transfected with plasmids expressing tagged CG2915, CG13427 CG1333 and CG6234 recombinant proteins. As controls for secreted, cytoplasmic, and transmembrane proteins, cells were transfected with constructs expressing V5-tagged Bip-GFP, β-Gal and Ptr fusion proteins, respectively. (A) Transfected cells were fractionated to obtain high-speed pellets (P) and supernatants (S). Equivalent volumes of cell fractions plus the medium in which cells were grown (M) were loaded on the gel and subjected to western blot analysis with anti-V5 (panels a and b and d to g) or anti-myc (panel c). (B) Transfected S2R+ cells were stained with anti-V5 (green in a and b and d to g) or anti-myc (green in c) and phalloidin to visualize actin (red in panels a to g).

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