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Figure 7 | BMC Biology

Figure 7

From: scribblemutants promote aPKC and JNK-dependent epithelial neoplasia independently of Crumbs

Figure 7

aPKC CAAXDN restrains scrib 1 + N ACT neoplastic overgrowth. Larval eye/antennal imaginal discs containing eyFLP-induced MARCM clones (green) at day 5 (A, B, E, G-H) and day 9 (C, D, F). Grey scale is Elav and Red is Phalloidin to mark F-actin. (A, C) UAS-N intra FRT82B scrib 1. Expression of NACT in scrib mutant clones results in large tumours at day 5 (A) and these become massive (compare to FRT82B control clones in E) and fuse with the brain lobes (bl) by day 9 (C). (B, D, F) UAS-N intra; FRT82B scrib 1 UAS-DaPKC CAAXDN. Co-expression of aPKCCAAXDN with NACT in scrib mutant clones fails to restore tumour differentiation but retards tumour overgrowth at day 5 (B compared to A), and this becomes more apparent by day 9 (D compared to C), although tumour cells are still observed between the brain lobes (arrows in F). (G) UAS-N intra; FRT82B UAS-DaPKC CAAXDN. Co-expression aPKCCAAXDN with NACT does not abrogate NACT-driven overgrowth of the eye/antennal disc. (H) UAS-N intra; FRT82B scrib 1 UAS-bsk DN UAS-DaPKC CAAXDN. Expression of aPKCCAAXDN and BskDN with NACT in scrib mutant clones prevents neoplastic tumor overgrowth and restores the characteristically overgrown mosaic discs of NACT-expressing clones.

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