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Figure 2 | BMC Biology

Figure 2

From: A genome-wide synthetic dosage lethality screen reveals multiple pathways that require the functioning of ubiquitin-binding proteins Rad23 and Dsk2

Figure 2

Identification of yeast genes that cause stronger growth retardation in rad23 dsk2 mutant cells than in wild-type cells. (A) Representative images from plasmid overexpression screen. Each of approximately 5280 yeast ORFs regulated by a galactose inducible Gal1 promoter was transformed separately into wild-type Y8835 and rad23Δ dsk2Δ mutant cells. Transformants were grown on media containing either glucose (SD, expression off) or galactose (SG, expression on). Each ORF is represented by two spots on the plate to reduce false positives. After two days for SD plates and three days for SG plates, colony sizes were scored for possible hits. Boxed spots are YLL030C on each plate. Strains and growth conditions are labeled at the bottom of each image. (B) A spotting assay indicates 11 genes cause preferential slow growth in rad23Δ dsk2Δ mutant cells. The spotting assay was conducted as described in Figure 1A. The identities of these genes and the corresponding strains are listed to the left and right of the panels, respectively.

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