Figure 4
dCTCF does not affect establishment of the Dp(1;f)LJ9 imprint. All genotypes tested are y1zag53d/ Dp(1;f)LJ9 ; +/+ but differ in parental genotype. External control progeny (Ex. Control) are generated from parents carrying Dp(1;f)LJ9 that have never been exposed to a mutant dCTCF allele. Progeny generated from a parent carrying both the imprinted Dp(1;f)LJ9 mini-X chromosome and a mutant dCTCF allele test for effects on imprint establishment (Mat or Pat-Est. CTCF30), whereas parental siblings carrying Dp(1;f)LJ9 and wild type for CTCF serve as an internal control (Mat or Pat-Est. CTCF+). (a) Maternal establishment of the Dp(1;f)LJ9 imprint is not affected by CTCF30. No significant change in red pigment levels was detected between external control progeny (Ex. Control; n = 23), mothers carrying Dp(1;f)LJ9MAT, and CTCF30(Mat-Est. CTCF30; n = 10), and mothers carrying Dp(1;f)LJ9MAT and CTCF+ (Mat-Est. CTCF+; n = 10). (b) No phenotypic difference is present between progeny generated from Dp(1;f)LJ9 carrying mothers, either mutant (Mat-Est. CTCF30) or wild type (Mat-Est. CTCF+), for CTCF. (c) Paternal establishment of the Dp(1;f)LJ9 imprint is not affected by CTCF30. No significant change in red pigment levels was detected between external control (Ex. Control; n = 44), fathers carrying Dp(1;f)LJ9PAT and CTCF30(Pat-Est. CTCF30; n = 18), and fathers carrying Dp(1;f)LJ9PAT and CTCF+ (Pat-Est. CTCF+; n = 29). (d) No phenotypic difference is present between progeny generated from Dp(1;f)LJ9 carrying fathers, either mutant (Pat-Est. CTCF30) or wild type (Pat-Est. CTCF+) for dCTCF.