Quantitation of the effect of tumour necrosis factor (TNF)-α on discontinuous adherens factor, stress fibres and focal adhesions (FAs) in confluent and subconfluent human umbilical vein endothelial cells (HUVECs). (A) Quantitation of discontinuous junctions per cell with or without TNF-α stimulation. (B) Quantitation of actin filament staining from confocal images between confluent and subconfluent HUVECs with and without TNF-α stimulation as in Figure 4. (C) Quantitation of FA-like paxillin clusters in confluent and subconfluent HUVECs with and without TNF-α stimulation. Error bars indicate standard error of mean; n ≥ 3 experiments. *, P < 0.008; **, P < 0.001, compared to confluent unstimulated cells. AU, arbitrary units.