Figure 6
Differential effect of Mn2+ on superactivity in the BH-H N and BH-H C Region. The activities of the most superactive substitutions in BH-HN and BH-HC are compared in Mn2+ substituted reactions relative to the wildtype enzyme. The BH-HN substitutions (mjA' M808-D, -E and -P) continue to display a high degree of superactivity, whereas BH-HC substitutions (mjA' Q823-D, -E and S824-P) only transcribe at rates comparable to wildtype RNAP.