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Table 1 List of primers used for 3'RACE, real time polymerase chain reaction (PCR), multiplex PCR, genotyping, and northern probe preparation. All primer sequences are in 5'-3' direction.

From: Identification and characterization of Dlc1 isoforms in the mouse and study of the biological function of a single gene trapped isoform

Primers for 3'RACE

   

1st strand CDNA synthesis

CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGCTTTTTTTTTTTTTTTTT

 

Forward primers

Reverse primers

Sequencing primers

1st round PCR

CTATCAGAAAGAGGAGCTGGGAAG

AGACCGAACAGCCTTTTATTTCTG

ACGTCTTAGCAGCTCCTTATGTAG

ACAAAAGCACTCTCGGGGTCAG

AAACTGGACCAACTAGACCAAGAC

ATCTGAATTGCACAGGCAAAACAG

GCCCTAAGCCTTTCCAGATG

CTGCGCCGACCTTAATGTGTAG

CCAGTGAGCAGAGTGACG

 

2nd round PCR

CTATCAGAAAGAGGAGCTGGGAAG

AGACCGAACAGCCTTTTATTTCTG

ACGTCTTAGCAGCTCCTTATGTAG

ACAAAAGCACTCTCGGGGTCAG

AAACTGGACCAACTAGACCAAGAC

ATCTGAATTGCACAGGCAAAACAG

TCCTTCAGCGACCACATCAGAG

CGCCGACCTTAATGTGTAGAGAC

GAGGACTCGAGCTCAAGC

ATTTTTTTTTTTTTTTTTTTTT

GTTTTTTTTTTTTTTTTTTTTT

CTTTTTTTTTTTTTTTTTTTTT

& different forward primers

Primers for Northern probe

1st round PCR

CTGCGCCGACCTTAATGTGTAG

GTTGCAGTCACGGGTGCTTC

 

2nd round PCR

CTCGAGCTGCGCCGACCTTAATGTGTAG

GTCGACGTTGCAGTCACGGGTGCTTC

 

Primers for real time quantification of splice variants

7.6 Kb transcript

TCCACCGATCCATCATCCACTC

CGGTGAGGACTGATCTCCAGCTTCATG

 

6.2 Kb transcript

TCCTTCAGCGACCACATCAGAG

GACTGGTTTCCCCCAGTATGCA

 

6.1 Kb transcript

CGCCGACCTTAATGTGTAGAGAC

  

2.1 Kb transcript

TCCACCGATCCATCATCCACTC

GCCCACTGAGTGCTGAGACTG

AAGTAC

 

GAPDH

TTC CGT GTT CCT ACC CCC AAT GTGT

GGAGTTGCTGTTGAAGTCGCAGGAG

 

Primers for methylation study

6.1 Kb Transcript

Biotin-GTAAGAAAAGGTAGAGGGGAAATTGAGTA

CTCCTTCCCCCTTTCCTAAAATAT

TTTTCCCAAAATCTACACT

6.2 Kb transcript

TAGGAGGTTAGTATGGGTTGTA

Biotin-AAACCTAATACTAACATAAAACAATATC

AAATTGTTTTTTTGATTTTAA

Multiplex PCR primers for genotyping Dlc1 gene trap embryos

 

TCCTGCTGGTGTGTGTAAACAGAC

GTGTAAGTTTGCCTCCTCCTTTGG

 
 

GATTCCACCGCCGCCTTCTATG

TCAAGCAAAAAGGTCTCGTATGTTGTG

 

Multiplex PCR primers for fusion transcripts

(7.6+2.1)Kb transcript

TCCACCGATCCATCATCCACTC

ACGACGACAGTATCGGCCTCAG

 

6.2 Kb transcript

TCCTTCAGCGACCACATCAGAG

  

6.1 Kb transcript

CGCCGACCTTAATGTGTAGAGAC

 Â