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Figure 3 | BMC Biology

Figure 3

From: ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function

Figure 3

Expression patterns of human ankyrin repeat and suppressor of cytokine signalling (SOCS) box protein 9 (ASB9) and ASB9ΔSOCS. (a) The expression level of ASB9 mRNA was analysed by real-time PCR using the cDNAs from indicated human tissues. The mRNA levels were normalised using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as an internal control, and relative expression was determined by dividing all normalised values within a data set by the normalised arbitrary units of the control. (b) Expression of ASB9 in human cell lines and human peripheral blood mononuclear cells (hPBMCs). (c) Expression of ASB9 in human hepatoma cell lines and normal human hepatocytes. The expression of ASB9 mRNA and protein was analysed by means of reverse transcription (RT)-PCR and immunoblotting, respectively. The β-actin expression level was used as a control. (d) Expression of mAsb-9 in mouse cell lines and lymphoid tissues. The GAPDH expression level was used as an mRNA control. The expression of mAsb-9 protein in the mouse cell lines and primary cells was analysed by immunoblotting with anti-ASB9 antibody. The β-actin expression level was used as a protein control.

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