Figure 2

In situ hybridization of c-myc, sall4 and sox2 in developing and regenerating limbs and tails. A-A"') Expression of c-myc in developing st52 limbs (A) and amputated limbs at 0 dpa, 1 dpa and 3 dpa (A'-A"'). c-myc expression is diffuse and very weak in the developing limb bud and the blastema. B-B"') Expression of sall4 in developing st52 (B) and regenerating limbs at 0 dpa, 1 dpa, 3 dpa (B'-B"'). sall4 is expressed diffusely in the distal mesenchyme of the developing limb bud and after amputation in the whole of the blastema. C-C"') Expression of sox2 in developing (C) and regenerating (C'-C"') limbs. sox2 is expressed proximal to the blastema in the posterior mesenchyme. D) c-myc mRNA is expressed in the entire regeneration bud of a 3 dpa tail and is much stronger than in limbs. E) sall4 mRNA expression in 3 dpa tail is mainly seen in the distal tip of the regenerating tail. F) sox2 mRNA expression in 3 dpa tail is confined to the neural tube. (dpa = days post amputation). Thin black line indicates amputation plane