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Table 4 Discrimination against 2' and 3'-deoxy substrates by ΔTL RNA polymerase.

From: Stepwise mechanism for transcription fidelity

 

cATP(ECA)/

c2'dATP(ECA)

cCTP(ECC)/

c2'dCTP(ECC)

cGTP(ECG3)/

c2'dGTP(ECG2)

cUTP(ECU)/

TTP(ECU)

cATP(ECA)/

c3'dATP(ECA)

cGTP(ECG3)/

c3'dGTP(ECG2)

Discrimination,

folds

~6

~11

~3

~17

~3

~0.4

  1. Kinetic discrimination was quantified as a ratio of the rate of incorporation of 1 mM cNTP to the rate of misincorporation of 1 mM of a corresponding c2'dNTP or c3'dNTP in elongation complexes identified.