Skip to main content

Table 5 Contribution of individual fidelity steps.

From: Stepwise mechanism for transcription fidelity

Fidelity contribution

Open active centre

Trigger loop folding

Total

(kpol [cGTP]/Kd [cGTP])/(kpol [ncGTP]/Kd [ncGTP])

87

5950

518000

Kinetic kpol [cGTP]/kpol [ncGTP]

3.8

970

3700

Affinity Kd [ncGTP]/Kd [cGTP]

23

6.1

140

(kpol [cGTP]/Kd [cGTP])/(kpol [c2'dATP]/Kd [c2'dATP])

1.7

21000

36000

Kinetic kpol [cGTP]/kpol [c2'dATP]

1.2

1500

1800

Affinity Kd [c2'dATP]/Kd [cGTP]

1.4

14

20

(kpol [cGTP]/Kd [cGTP])/(kpol [c3'dATP]/Kd [c3'dATP])

0.96

2030

1990

Kinetic kpol [cGTP]/kpol [c3'dATP]

0.6

1180

710

Affinity Kd [c3'dATP]/Kd [cGTP]

1.6

1.8

2.8

  1. Values were quantified using data of Table 1: 'Open active centre' values were generated from the row identifier ratios using the ΔTL data; 'Total' values were generated from the row identifier ratios using the wild-type (WT) data; 'TL folding' values were generated from the row identifier ratios using the ratio of the WT ratio over the ΔTL ratio.