Figure 4
From: A role for NRAGE in NF-κB activation through the non-canonical BMP pathway
In vivo overexpression of NRAGE in the mouse kidney. A: Control staining of secondary antibodies in the NRAGE:Cherry transgenic mouse. B: Merged picture of NRAGE:Cherry and IKKα/β-phosphorylation. C: Merged picture of NRAGE:Cherry and NF-κB activation. Note the translocation of NF-κB to the nucleus denoted by teal colored nuclei. D: Merged picture of NRAGE:Cherry and MIF. E: Hematoxylin and Eosin staining of NRAGE:Cherry transgenic kidney. F: Control staining for secondary antibodies in the wild type mouse. G: Staining of IKKα/β-phosphorylation in the wild type mouse. H: Staining of NF-κB activation in the wild type mouse. I: Merged picture of NRAGE:cherry and MIF in the wild type mouse. J: Hematoxylin and Eosin staining of Wild-Type kidney. K-O: 63× magnification of C, red arrows point to nuclear localization of p65 as depicted by teal coloring.