Figure 4

Sequence alignment of nitrile hydratase leader peptides (NHLPs) from natural combinatorial biosynthetic clusters. ClustalW alignment [44] of NHLPs from a putative thiazole/oxazole-modified microcin cluster from (A)Pelotomaculum thermopropionicum SI (12 sequences) and (B) Azospirillum sp. B510 (eight sequences). Possible sites for thiazole formation are highlighted in cyan and sites of potential oxazole and methyloxazole formation are yellow. The locus tag is given to the left of the sequence and the amino acid position is given on the right. An asterisk implies an invariant residue, while the semicolon and period show positions that are highly and moderately related, respectively. Underlined red text indicates the putative leader peptide cleavage motif.