Figure 4

Binding of proline-rich region (PRR)/WWI-IV to the homologues of the E6-associated protein C-terminus (HECT) domain of atrophin-1-interacting protein 4 (AIP4) and wild-type (WT) spartin. (a) Binding isotherm of the glutathione S-transferase (GST)-HECT protein to PRR/WWI-IV. AU = arbitrary units. (b) Binding isotherm of the GST-WT spartin 155-367 protein to PRR/WWI-IV. (c) Inhibition of GST-HECT binding to PRR/WWI-IV by WT spartin. All experiments were repeated three times. Data are shown as mean ± SD with an R² ≥ 0.95.