Figure 3

Female sexual attraction to urine fractions separated by anion exchange chromatography containing different major urinary proteins (MUPs). Total time spent under urine stimuli from B6 males (blue bars) and from matched females (pink bars), together with the difference in time spent under male minus female fraction (circles), plotted as means ± standard error of mean. Significant P values indicate greater attraction to the male stimulus (matched pair t-tests of log transformed data (t) or Wilcoxon matched pair tests (z) when log transformed data did not approximate normality); n.s. = no significant attraction to male. (A) Response to urine fractions containing different MUPs (separation shown in Figure 2). I: 18645Da MUP (in male fraction only); II: 18709Da MUP (both sexes); III: 18694Da and 18713Da MUPs (both sexes); IV: 18893Da MUP (darcin, male only). The protein concentration in each male and female fraction is given in Table 2. (B) Male fractions I and IV were subsequently tested against female fraction III. For each test n = 12 except fraction I which bordered on significance and sample size was increased to n = 17.