Serious limitations of the QTL/Microarray approach for QTL gene discovery

Table 1 Published studies analyzing parental strains using the QTL/Microarray approach in rat

	Phenotype	QTL Mapping		Microarray Experiment			References
		Design	Strains	Design	Tissues	Platform
	*Targeted custom arrays*
	Hypertension	Congenic	DS, LEW	Congenic	Kidney	Custom/ TIGR	[43]
	Hypertension	F2	DS, LEW	Parental	Kidney	RT-PCR	[31]
	*Whole-Genome arrays*
•	Aerobic running capacity	Congenic	DS, DR	Parental	Kidney	RGU34	[34, 95, 96]
	Alcohol addiction	Congenic	iP, iNP	Congenic	NAC, FC, ABR, hippocampus, striatum	RG230v2	[97]
	Hypertension	F2	SBH/y, SBN/y	Parental	Kidney	RAE230	[41, 98]
•	Hypertension	Congenic	DS, LEW	Congenic	Kidney	RGU34	[30]
•	Hypertension	Congenic	DS, DR	Congenic	Kidney	RGU34	[42, 99]
•	Hypertension	Congenic	SHRSP, WKY	Congenic	Kidney	RGU34	[44]
	Hypertension	F2	DS, LEW	Parental	Kidney	RAE230	[32]
	Hypertension	F2	DS, LEW	Parental	Kidney, adrenal gland, liver, brain	RG230v2	[33]
•	Kidney stones	Congenic	GHS, WKY	Congenic	Kidney, SI, FOC	RAE230	[37]
	Metabolic syndrome	Congenic	SHR, WKY	Congenic	EWAT	Spotted	[27]
	Hypertension	Congenic	DS, DR	Parental	Kidney	GF 300	[40]

Tissue abbreviations:ABR: amigdala brain region, EWAT: Epididymal white adipose tissue, FC: frontal cortex, FOC: femoral osteoclast cells, NAC: nucleus accumbens, SI: small intestine. Strain abbreviations:DS: Dahl salt-sensitive, DR: Dahl salt-resistant, GHS: genetic hypercalciuric stone-forming, iP: alcohol non-preferring, LEW: Lewis rats, P: alcohol preferring, SHR: spontaneously hypertensive rat, SHRSP: stroke-prone spontaneously hypertensive rat, SBH/y: Sabra salt-sensitive hypertensive, SBN/y: Sabra salt-resistant normotensive, WKY: Wistar Kyoto.
• Articles included in Table 3.

ISSN: 1741-7007