Figure 5From: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleusEvc2 is required for the localization of Evc at the base of primary cilia. (a). RT-PCR amplification products from Evc2 null (-/-) and wild-type (+/+) MEFs. As expected, no Evc2 transcript was detected in the Evc2 null MEFs. A significant amount of Evc transcript was amplified in the Evc2 null MEF sample. Hprt transcript was amplified as a control. (b). Western blot analysis of Evc protein in Evc2 null MEFs. The amount of β-actin detected on the same blot was used as a loading control. Evc (approximately 130 kDa) is present in Evc2 null (-/-) MEFs despite having reduced levels (approximately 50%) (c). Representative immunofluorescent staining of Evc in MEF cells. Despite the presence of protein, Evc (red) was not detected at the base of primary cilia in Evc2 null MEFs (-/-). Primary cilia were identified by the presence of acetylated tubulin (green) and nuclei stained with DAPI (blue). Scale bar 10 μm.Back to article page