A next-generation sequencing method for overcoming the multiple gene copy problem in polyploid phylogenetics, applied to Poa grasses

Table 2 Ecological association and herbarium accessions used for each Poa species

Species	Ecological association	Herbarium accessions (all CANB)
		Victoria	New South Wales	Tasmania
Poa annua* (5)	Subalpine, montane, elsewhere		312513, 343723, 407148
P. clivicola (8)	Alpine, subalpine		228786, 228805, 329728, 438760, 647153, 7901040, 8702739, 8906309
P. costiniana (8)	Alpine	402908**	228898, 505348, 543047**, 619540, 619578, 8101033, 9707880
P. ensiformis (5)	Subalpine, montane	48084, 67340, 363091	645156, 8906370
P. fawcettiae (10)	Alpine	67067, 488116, 9501151	228865, 228879, 230925, 345640, 505340, 505353, 505353
P. gunnii (5)	Alpine, subalpine	Not present	Not present	195943, 341316, 402406, 8904303, 9215410
P. helmsii (6)	Alpine, subalpine		68026, 228839, 312845, 644862, 8316158, 9301326
P. hiemata (5)	Alpine	8500047	343721, 506031, 7705690	9012247
P. hothamensis var. hothamensis (6)	Alpine, subalpine	67341, 207890**, 207886, 207893, 409990	Not present	Not present
P. phillipsiana (6)	Alpine, subalpine	67342, 207889	615444, 651518, 8413383
P. sieberiana var. sieberiana (1)	Alpine, subalpine, montane, elsewhere		780260

Herbarium accession numbers are listed by Australian state of origin. Total number of accessions used is given in brackets after the species name.
* indicates the introduced species Poa annua, used as an outgroup
** indicates specimens that were replicated from the DNA extraction stage onwards to check for replicability

ISSN: 1741-7007