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Figure 6 | BMC Biology

Figure 6

From: Real-time visualization of heterotrimeric G protein Gq activation in living cells

Figure 6

Fluorescence lifetime data. Average cyan fluorescent protein (CFP) lifetimes measured under different conditions. The phase lifetime of Gαq-mTqΔ6 was measured in the absence (n = 15) and presence (n = 12) of the acceptor YFP-Gγ2. The difference in lifetime was significant. The Gq sensor was coexpressed with the histamine H1 receptor (H1R) and CFP lifetimes were measured before and after addition of 100 μmol/l histamine (n = 6). The Gq sensor was coexpressed with the guanine nucleotide exchange factor p63RhoGEF and CFP lifetimes were measured before and after addition of 100 μmol/l histamine (n = 6). In both conditions the lifetime difference before and after stimulation was significant. The CFP lifetimes before addition of histamine are not different (Student t-test, 95% CI) in the presence or absence of either H1R or p63RhoGEF.

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