Figure 3
Impact of autophagic regulators on autophagosomal fusion/degradation and endolysosomal turnover. mCherry-green fluorescent protein (GFP) (tandem)-microtubule-associated protein 1 light chain 3 B (LC3) (a,b) or GFP-Rab7 (c,d) cells were exposed to either full medium (FM) (a,c) or nutrient deprivation (ND) (b,d) conditions ± respective drugs (rapamycin, 0.1 μM; AICAR, 200 μM; resveratrol, 100 μM; bafilomycin A1 (Baf), 0.1 μM; wortmannin (WM), 2.3 μM; 3-MA, 5 mM) for 6 h. Fluorescence intensities of tandem-LC3/GFP-Rab7 were detected by flow cytometry. Data was normalized as described in Materials and methods (including normalization to control (Ctr) constructs). Values represent fold changes in relation to the respective control condition (FM for (a,c) and ND for (b,d)). *P < 0.05, **P < 0.01, ***P < 0.001.