Figure 4

Selected PMMs have different effects on the actin cytoskeleton and migration. PC3 cells were transfected with the indicated siRNA pools for each PMM. (A) After 24 h, cells were seeded at subconfluence on coverslips, then fixed after 48 h and stained for F-actin (red) and α-tubulin (green). Scale bar, 100 μm. (B) Cells were seeded on plastic dishes and monitored by time-lapse microscopy for 14 h. Cells were tracked using ImageJ software (Chemotaxis and Migration Tool plugin). Tracks for 25 cells randomly selected from 70 to 99 cell tracks from three different movies for each PMM are shown.