Figure 5

FAM40A and FAM40B have opposite effects on cell shape and migration. PC3 cells were transfected with the siRNA pools targeting FAM40A, FAM40B or control siRNA. (A) After 24 h, cells were seeded at subconfluence on coverslips, then fixed after 48 h and stained for F-actin (red) and tubulin (green). (B) Cells were seeded on plastic dishes and monitored by time-lapse microscopy for 14 h. Cells were tracked using ImageJ software (Chemotaxis and Migration Tool plugin). Tracks for 25 cells randomly selected from 70 to 99 cell tracks from three different movies for each of control, FAM40A and FAM40B are shown. (C) The circularity of cells in (A) was determined using ImageJ (Circularity plugin). A circularity value of 1.0 indicates a perfect circle. As the value approaches 0, it indicates an increasingly elongated polygon.