Figure 5

Effect of Hes5-1 3'UTR on the expression of the VNP reporter protein. (A-I) Analysis of VNP and CherryNLS expression in sections of embryos co-electroporated with either pCAG-VNPΔ3UTR and pCAG-CherryNLS (A-C), pCAG-VNP and pCAG-CherryNLS (D-F), or pCAG-VNPΔ3UTR20 and pCAG-CherryNLS (G-I), shows that the presence of the 3'UTR of Hes5-1 diminishes the expression of VNP and that the conserved 20 bp region in the 3'UTRs of Hes5 genes has a significant contribution to this effect. DAPI (blue) was used for nuclear counterstaining in C, F and I. (J) Chart showing the percentage of VNP expression from the different reporter constructs relative to that of pCAG-VNPΔ3UTR. Statistical analysis was done using t-test; *p-value < 0.002; **p-value < 0.0002.