Figure 1

Non-random mapping of small RNAs (tRFs) on tRNA genes (HEK293 human cell line). (A) Numbers of unique tRFs that were present at a minimum of 20 reads per million are plotted against length of the tRF. (B) Length distribution of reads that mapped to a specific tRF-5 (GlyGCC), tRF-3 (ValCAC) and tRF-1 of (LeuTAG). (C) Illustration of a mature tRNA showing the cut sites that would generate the different subclasses of tRF-5s and tRF-3s.