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Figure 2 | BMC Biology

Figure 2

From: The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor

Figure 2

Circulating yolk sac-derived cells from HH16 to HH17 stage embryos can be differentiated into macrophages in vitro . (A, B) Twenty microliters of whole blood from five HH16 to HH17 stage embryos was cultured with (A) or without (B) 350 ng/ml chicken CSF1-Fc for 14 days. Note the confluent monolayer of cells in panel A, and the absence of any surviving cells in panel B. 20× magnification. (C) Shows the ability of cells derived by cultivation from EGFP+ yolk sac donors to internalize fluorescent zymosan particles. (D, G) Shows the same cell populations immunostained with control (D), anti-CSF1R (E) anti-KUL01 (F) or anti-MHCII (G). Scale bars = 10 μm.

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