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Figure 3 | BMC Biology

Figure 3

From: Expression of leukemia inhibitory factor in Müller glia cells is regulated by a redox-dependent mRNA stability mechanism

Figure 3

H2O2-induced stabilization of Lif in rMC-1 and primary Müller cells is independent of p38 MAPK. A) rMC-1 cells were (+) or were not (−) treated with p38 MAPK inhibitor SB202190 (SB) in the presence (+) or absence (−) of H2O2 (50 μM) for one hour. RNA levels were determined by real-time PCR and expressed relative to the levels in untreated cells. Shown are means ± SEM of N = 6 (five independent experiments). One-way ANOVA with Tukey’s posttests were used to compare all pairs of columns. B) Primary mouse Müller cells isolated from Rlbp-GFP mice heterozygous (Ttp +/−) or homozygous (Ttp −/−) for the Ttp knockout allele were (+) or were not (−) serum deprived (SD) for two hours in the presence (+) or absence (−) of H2O2 (50 μM). Lif mRNA levels were determined by real-time PCR at two hours of treatment and expressed relative to the levels of untreated cells. Shown are means ± SEM of N = 3 to 4. One-way ANOVA with Sidak’s posttests were used to compare the effect of treatment for both Ttp +/− and Ttp −/− Müller cells. (**) P <0.01 and (***) P <0.001. ANOVA, analysis of variance; SEM, standard error of the mean.

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