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Figure 5 | BMC Biology

Figure 5

From: Expression of leukemia inhibitory factor in Müller glia cells is regulated by a redox-dependent mRNA stability mechanism

Figure 5

AREs are involved in cis-regulation of Lif mRNA stability in rMC-1 Müller cells. A) rMC-1 cells were transfected with reporter constructs fused to wild type or mutated R27 sequences (see Additional file 4: Figure S4A), as indicated. B) rMC-1 cells were transfected with reporter constructs fused to R27 sequences containing increasing numbers of AREs (see Additional file 4: Figure S4B), as indicated. C) rMC-1 cells were transfected with reporter constructs fused to indicated wild type or mutated R36 sequences (see Additional file 4: Figure S4C), as indicated. Luminescence levels were always determined after 24 hours and shown relative to the level generated by the ΔUTR control construct. Shown are means ± SEM of N = 3 to 4. Squares in horizontal bars indicate AREs or an miR-29 binding site, respectively, in the analyzed sequences. One-way ANOVA with Dunnett’s posttests were used to compare expression levels to constructs containing the respective wild type sequence (A, C) or to ‘2 ARE’ (B). (*) P <0.05, (**) P <0.01, (***) P <0.001 and (****) P <0.0001. ANOVA, analysis of variance; AREs, AU-rich elements; SEM, standard error of the mean.

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