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Figure 7 | BMC Biology

Figure 7

From: Expression of leukemia inhibitory factor in Müller glia cells is regulated by a redox-dependent mRNA stability mechanism

Figure 7

Silencing of Khsrp reduces Lif mRNA levels in non-stressed rMC-1 Müller cells. A, B) rMC-1 cells were transfected with siRNAs directed against Khsrp. Scrambled siRNA was used as control (Ctrl). Medium was changed 48 hours after transfection and RNA levels for Khsrp (A) or Lif (B) were determined after two hours by real-time PCR. C) rMC-1 cells were transfected with siRNAs directed against Khsrp. Scrambled siRNA was used as control (Ctrl). Medium was changed 48 hours after transfection and cells were (SD) or were not (C) serum deprived. H2O2 (50 μM) was added to samples as indicated. RNA levels were determined by real-time PCR two hours after treatment and expressed relative to untreated cells (C), which were set to 1 for each knockdown series. Two different siRNAs (Khsrp (si1), Khsrp (si2)) were used to exclude off-target effects. Shown are means ± SEM of N = 3 to 4. Student’s t-test was used to compare downregulation of Khsrp and Lif in untreated cells (A, B). One-way ANOVA with Dunnett’s posttests were used to compare Lif or Khsrp levels to controls (A, B) or Lif levels after SD + H2O2 treatments (C). (***) P <0.001. ANOVA, analysis of variance; SEM, standard error of the mean.

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