Table 1 Vector list of cloned regions used in constructs
Vector name | Lif 3′UTR position (1 to 3180) | Length of 3′UTR (bp) | AREs | Sequence source | Cloning method |
|---|---|---|---|---|---|
∆UTR | 0 | 0 | 0 | NA | RE |
Whole Lif 3′UTR | 1 to 3180 | 3180 | 11 | mus musculus | PCR/RE |
hgen DNA | NA | 3142 | 2 | homo sapiens | PCR/RE |
AU-rich R I | 1 to 570 | 570 | 4 | mus musculus | PCR/RE |
AU-rich R II | 2556 to 3180 | 625 | 5 | mus musculus | RE |
hACTB 3′UTR | NA | 600 | 3 | homo sapiens | PCR/RE |
R27 (wt) | 2999 to 3180 | 182 | 3 | mus musculus | RE |
R32 | 2805 to 2998 | 194 | 0 | mus musculus | PCR/RE |
R31 | 2541 to 2812 | 272 | 2 | mus musculus | PCR/RE |
R38 | 2079 to 2245 | 167 | 0 | mus musculus | PCR/RE |
R36 (wt) | 1062 to 1231 | 170 | 2 | mus musculus | PCR/RE |
R35 | 398 to 570 | 173 | 4 | mus musculus | PCR/RE |
R37 | 62 to 229 | 168 | 0 | mus musculus | PCR/RE |
R27-A | 2999 to 3180 | 182 | 1 | mus musculus | SD |
R27-B | 2999 to 3180 | 182 | 1 | mus musculus | RE/AN |
R27-C | 2999 to 3180 | 182 | 1 | mus musculus | SD |
R27-No | 2999 to 3180 | 182 | 0 | mus musculus | SD |
R36-DE | 1062 to 1231 | 170 | 2 | mus musculus | SD |
R36-D | 1062 to 1231 | 170 | 1 | mus musculus | SD |
R36-E | 1062 to 1231 | 170 | 1 | mus musculus | SD |
R36-No | 1062 to 1231 | 170 | 0 | mus musculus | SD |
R27 2 ARE | 2999 to 3180 | 182 | 2 | mus musculus | SD |
R27 4 ARE | 2999 to 3180 | 237 | 4 | mus musculus | SD |
R27 9 ARE | 2999 to 3180 | 351 | 9 | mus musculus | SD |