The study of Priapulus caudatus reveals conserved molecular patterning underlying different gut morphogenesis in the Ecdysozoa
© Martín-Durán and Hejnol; licensee BioMed Central. 2015
Received: 26 November 2014
Accepted: 13 April 2015
Published: 21 April 2015
The digestive systems of animals can become highly specialized in response to their exploration and occupation of new ecological niches. Although studies on different animals have revealed commonalities in gut formation, the model systems Caenorhabditis elegans and Drosophila melanogaster, which belong to the invertebrate group Ecdysozoa, exhibit remarkable deviations in how their intestines develop. Their morphological and developmental idiosyncrasies have hindered reconstructions of ancestral gut characters for the Ecdysozoa, and limit comparisons with vertebrate models. In this respect, the phylogenetic position, and slow evolving morphological and molecular characters of marine priapulid worms advance them as a key group to decipher evolutionary events that occurred in the lineages leading to C. elegans and D. melanogaster.
In the priapulid Priapulus caudatus, the gut consists of an ectodermal foregut and anus, and a mid region of at least partial endodermal origin. The inner gut develops into a 16-cell primordium devoid of visceral musculature, arranged in three mid tetrads and two posterior duplets. The mouth invaginates ventrally and shifts to a terminal anterior position as the ventral anterior ectoderm differentially proliferates. Contraction of the musculature occurs as the head region retracts into the trunk and resolves the definitive larval body plan. Despite obvious developmental differences with C. elegans and D. melanogaster, the expression in P. caudatus of the gut-related candidate genes NK2.1, foxQ2, FGF8/17/18, GATA456, HNF4, wnt1, and evx demonstrate three distinct evolutionarily conserved molecular profiles that correlate with morphologically identified sub-regions of the gut.
The comparative analysis of priapulid development suggests that a midgut formed by a single endodermal population of vegetal cells, a ventral mouth, and the blastoporal origin of the anus are ancestral features in the Ecdysozoa. Our molecular data on P. caudatus reveal a conserved ecdysozoan gut-patterning program and demonstrates that extreme morphological divergence has not been accompanied by major molecular innovations in transcriptional regulators during digestive system evolution in the Ecdysozoa. Our data help us understand the origins of the ecdysozoan body plan, including those of C. elegans and D. melanogaster, and this is critical for comparisons between these two prominent model systems and their vertebrate counterparts.
A defining character of animals is the need to incorporate other organisms, or their products, for nourishment. Although different strategies have evolved to accomplish this task [1,2], the solution present in almost all metazoans is the development of organs with specialized cell types to ingest and digest food, and absorb the resulting nutrients. The digestive system is thus a central morphological and physiological constituent of metazoans, and, as such, has experienced intense adaptation and diversification, as animals have radiated into different ecological niches and utilized new food sources and predatory strategies . Accordingly, how this variety of digestive systems originated emerges as a key question in the study of animal body plan evolution.
Whereas many early-branching animal lineages, such as Cnidaria (that is, jellyfish, corals), show a sack-like intestine that opens to the exterior through the mouth, most bilaterally symmetrical animals (for example, mammals, flies, and earthworms) exhibit a through gut with two openings, the mouth and the anus, and distinct regions specialized for particular feeding tasks . Pharynxes, jaws, and proboscides to capture and grind food, stomachs and digestive glands to process nutrients, and cloacae to release excretory products are just a few examples of the specializations exhibited by animal digestive systems. Despite this diversity in gut architecture and complexity, the comparative study of different bilaterian animals has revealed commonalities in the early ontogenetic stages of gut formation, and a handful of genes have been related to the specification and initial development of the digestive system [3-6]. The gut usually forms from a population of cells that are localized at one point of the early embryo and that get internalized in a process called gastrulation . These cells, the endoderm (literally, internal skin) of the embryo, form the most medial part of the intestine, which opens into the ectoderm (external skin) through the mouth and the anus. Beyond these broad commonalities, the way in which the gut forms may significantly change as organisms undergo developmental adaption in response to de novo habitat colonization [8-10].
Most recent phylogenies place the exclusively marine priapulid worms (Priapulida), and the related taxa kinorhynchs and (likely) loriciferans, as the earliest branching ecdysozoan lineage (Scalidophora), and thus the sister group to the remaining ecdysozoans, including nematodes and arthropods [15,17,29] (Figure 1A). The extant Priapulida comprise only 19 described species [2,30], but were among the most abundant and widespread animals in the Early Cambrian . The oldest trace fossils from the beginning of the Cambrian (Treptichnus pedum) resemble burrowing priapulids, or morphologically very similar animals . Priapulids, commonly referred to as penis worms, are large sized (0.5 to 20 cm), mud-dwelling or interstitial annulated worms, with an anterior proboscis (or introvert), and a terminal mouth [2,33] (Figure 1B). Reports on their embryonic development are scarce and mostly focused on the early stages of development of the species Priapulus caudatus Lamarck 1816 [34,35]. P. caudatus reproduces by external fertilization, and the small embryos undergo holoblastic radial cleavage, gastrulation by invagination and epiboly , and deuterostomic formation of the mouth , which are all considered to be plesiomorphic features in the Ecdysozoa [34,36]. This combination of characters, together with their slow rate of molecular evolution , render the Priapulida, and in particular the representative species P. caudatus, as the key conservatively evolving ecdysozoan group to compare with nematodes and arthropods, and to thereby infer ancestral characters for these species-rich lineages of animals.
In the present study, our aim was to characterize the formation of the gut in P. caudatus and then, by comparing our data with the knowledge on C. elegans, D. melanogaster, and other bilaterians, to decipher the evolutionary events that occurred after cladogenesis of the nematode and arthropod lineages. Principally, we focused on the morphological development of the endoderm into the definitive intestine, as well as on how the mesoderm segregates from the endoderm and its putative influence on the formation of the gut. We then analyzed mouth and head development, as well as the molecular regionalization of the definitive digestive system, by studying the expression of the mouth markers NK2.1, foxQ2, and FGF8/17/18; the midgut markers GATA456 and hepatocyte nuclear factor 4 (HNF4); and the hindgut markers wnt1 and even-skipped (evx). Our data shed light on the origins and evolution of the digestive tracts of C. elegans, D. melanogaster, and the Ecdysozoa in general. Importantly, our data demonstrate that a conserved molecular patterning system underlies the great variability of ontogenetic modes and architectures observed in the digestive systems of ecdysozoans.
Gut formation in P. caudatus
After the formation of the gut anlage, about days 7 to 8 of development, the introvert retracts and becomes sheathed in the trunk (Figure 2C,D,C’,D’). This is a key event during priapulid embryogenesis, as it results in the emergence of the larval/adult body plan . Strikingly, when the introvert develops it is unfolded (Additional file 3: Figure S2). The animal-most ectoderm corresponds to the inner epidermis of the introvert, often called the oral or buccal cavity. At the most anterior region of the oral cavity, which in the embryo corresponds to the anterior region of the introvert-trunk boundary, the scalids (feeding teeth) develop ( and Figure 2B). The ectodermal indentation of the introvert-trunk boundary thus corresponds to the external epidermis of the introvert, the neck region (transition from the introvert and trunk), and the anterior epidermis of the trunk. During retraction, the initially extended inner gut (Figure 2C) is pulled down to the posterior end of the embryo (Figure 2D), as the introvert is incorporated inside the trunk, which also extends anteriorly during this process. As a result, the foregut, located at first at the anterior pole of the embryo, is internalized inside the embryo, and adopts a posterior position within the now folded introvert (Figure 2D, D’; Additional file 3: Figure S2). The posterior region of the embryo, and thus the anus, is not significantly affected by these major morphological rearrangements (Figure 2C,D,C’,D’). Additionally, introvert retraction is required for embryo hatching. The protrusion of the introvert eventually opens the hatching cap , allowing the hatching larva to escape.
A previous study of the external morphology of the hatching larva of P. caudatus reported the lack of mouth and anal openings in the larval cuticle . Despite this absence, the hatching larva does show a fully developed digestive tract (Figure 2E,E’), similar to the one observed during embryonic development. No additional glands or attached organs are observed in close contact with the tube-like intestine. The first molting event, which results in the formation of the first lorica larva , involves a significant change in larval morphology and cell number (Figure 2F). The introvert and trunk grow in size and complexity, the internal portion of the alimentary canal is now formed by a greater number of cells, and the mouth and anal openings are present in the cuticle . This observation suggests that the attainment of the mature digestive tract, as observed in the adult, is accomplished through successive molting events.
Mesoderm development in P. caudatus
Segregation of endodermal and mesodermal precursors from a common endomesodermal germ layer is the first step in the development of their respective cell types and organs. During and immediately after gastrulation in P. caudatus, the endomesoderm shows no overt signs of segregation between endodermal and mesodermal populations (Figure 2A). However, there is expression of the endodermal marker foxA in the most animally located endomesodermal cells . To identify the mesodermal precursors at this developmental stage, we analyzed the expression of the evolutionarily conserved mesodermal marker twist (twi) [40,41]. During gastrulation, twi transcripts are detected in the blastopore and the most vegetal endomesodermal cells, as well as in two lateral rows of internal cells (Figure 3A,B). Endoderm and mesoderm are thus likely distinct cellular populations already during gastrulation. As organogenesis proceeds through the introvertula stage, twi expression is detected in two broad rings of cells around the introvert and trunk (Figure 3C,D), which might correspond to the developing musculature (compare with phallacidin-positive muscles of the trunk and introvert in Figure 3F,G).
Differentiation of the mesoderm, and in particular of the surrounding visceral musculature, is essential for proper endoderm development in model organisms such as D. melanogaster and vertebrate embryos [4,28]. In P. caudatus, the organization of a recognizable gut tract by day 6 of development occurs simultaneously with the onset of muscle differentiation (Figure 3E,F). The first signs of this event are observed at the time of mouth formation, with the appearance of actin-positive circular fibers around the introvert-trunk boundary (Figure 3E). At the introvertula stage (Figure 3F), the body-wall musculature is obvious, with the development of circular muscles, mostly concentrated at the trunk level, and longitudinal muscles that connect the developing introvert with the trunk (inset Figure 3F). Before the retraction of the introvert (Figure 3G), the musculature appears further developed, in particular there are more muscle fibers at the introvert level. Introvert retraction, and thus the positioning of the digestive system in its final location, might be a muscle-controlled process, as is also the case during the protrusion and retraction of the adult introvert. As a consequence of the retraction of the introvert, the trunk musculature extends, and the circular packs of musculature and long retractor muscles become evident (Figure 3H). There are also shorter longitudinal retractor muscles connecting the posterior region of the introvert to the trunk. As observed with the digestive system, the musculature pattern observed in late embryos is conserved in the hatching larva (Figure 3I), and the number of muscle fibers increases after the first molting event (Figure 3J). Despite the fact that the adult priapulid gut is surrounded by a layer of longitudinal muscles that directly attaches to the basal lamina of the endoderm, our investigations point towards the absence of this musculature in priapulid embryos and first larval stages (see Figure 2E,F). The visceral musculature may thus develop in subsequent larval stages, in connection with the appearance of feeding behaviors  and a functional digestive system.
Cell proliferation and cell migration during mouth development
Before mouth invagination, cell proliferation is mostly concentrated in the animal hemisphere of the embryo (Figure 4B), in the region that will form the introvert. This observation explains the greater number of nuclei observed in the introvert region using standard nuclear staining methods (for example, compare introvert and trunk regions in Figure 2B,C), and this region corresponds to the area of brain and proboscis formation. Localization of EdU-positive cells at 12 and 24 hours after the initial pulse demonstrated that labeled cells remained at the introvert region (Figure 4C,D), and that the mouth is formed by cells that originate in the animal hemisphere (inset in Figure 4D). Once the mouth invaginates on the ventral side of the embryo (Figure 4E, and inset), proliferation appears mostly concentrated on one side of the introvert, in a three- to four-cell-wide stripe that spans from the base of the introvert to almost the most anterior tip of the embryo. Individual proliferative cells are also observed in different parts of the introvert and trunk. Labeling for EdU-positive cells, together with cells expressing the oral marker foxA , showed that these populations are co-localized (Figure 4F, inset; Additional file 4: Figure S3), and indicates that the asymmetric proliferation observed in the introvert at this stage occurs ventrally, at the region of mouth formation and nervous system development . At this stage, nuclei distribute more or less equally throughout the introvert ectoderm, except around the mouth and in the ventral midline where EdU-positive cells occur (Additional file 4: Figure S3), and ectodermal cells exhibit roughly the same size (see introvert region in Additional file 2: Video S1). Finally, cell proliferation decreases with the establishment of the basic body plan in the priapulid embryo after days 5.5 to 6 of development (Figure 4H-J), with only individual EdU-positive cells being observed in the introvert and trunk region after this time. Altogether, these results indicate that asymmetric cell proliferation is likely to be an important factor in the migration of the mouth from a ventral to an anterior terminal position, although they do not rule out that other factors also contribute to a certain extent. Additionally, the similar distribution of labeled cells at different time-points after a common EdU pulse suggests that cell migration is not a major force driving morphogenesis during P. caudatus development, as is also observed in the nematode C. elegans .
Anteroposterior patterning of the digestive tract of P. caudatus
The oral ectoderm marker NK2.1  is expressed on one side of the gastrula, separate from the blastopore (Figure 5A,B). At the introvertula stage, NK2.1 is expressed in the most apical region of the introvert, where the mouth is located (Figure 5C,D). foxQ2 is a conserved marker of apical neural ectoderm [44,45], and in C. elegans and D. melanogaster it is also expressed in the foregut [46,47]. During gastrulation, foxQ2 is expressed in the animal-most ectoderm, lateral ectoderm, and weakly in the ectoderm around the blastopore (Figure 5E,F). With the formation of the basic body plan at the introvertula stage, foxQ2 becomes expressed around the mouth and on one side of the introvert, presumably the ventral side - which is also the case for the neural marker otx . Finally, FGF8/17/18 shows conserved expression at the mouth region in many studied bilaterians [48,49], and is detected in the animal hemisphere during gastrulation in P. caudatus (Figure 5I,J). At the introvertula stage, FGF8/17/18 is expressed in the mouth and anus, as well as in six clusters of cells in the introvert, distributed in two bilaterally symmetrical rows of three clusters each (Figure 5K,L).
Orthologs of the GATA456 subfamily and HNF4 are evolutionarily conserved markers of the developing midgut . Neither marker was detected at the blastula stage in P. caudatus (Figure 5M,N,Q,R), and their expression only became evident at the introvertula stage, in the inner cells right below the mouth, and thus presumably in the developing midgut (Figure 5O,P,S,T).
Finally, wnt1 is a conserved marker of posterior regions across the Bilateria . During gastrulation, wnt1 is expressed vegetally, around the blastopore (Figure 5U,V), and this expression pattern remains at the introvertula stage, when wnt1 is detected in the posterior tip of the trunk, and anus (Figure 5W,X). The homeobox-containing gene evx has been shown to play a conserved role in patterning the posterior regions of bilaterian embryos [51,52]. At the gastrula stage, evx is expressed broadly at the vegetal pole (Figure 5Y,Z), and as observed with wnt1, its expression becomes reduced to the posterior end of the trunk and anus at the introvertula stage (Figure 5AA,AB).
Gut development in P. caudatus, and the ancestral state for the Ecdysozoa
The organogenesis of a through gut from the primordial endodermal cells also varies among different ecdysozoan lineages. In C. elegans, the formation of the midgut occurs from a 16-cell primordium made of eight tiers of two cells each . In this primordium, apical-basal cell polarization, lumen formation, and axial differentiation take place. The definitive midgut of the first larval stage strictly consists of 20 cells, and a similar olygocytose condition is observed in other members of the order Rhabditida and related taxa . However, the majority of adult nematodes exhibit an intestine with hundreds or thousands of cells, which develops from a large midgut rudiment , and thus the situation observed in C. elegans is likely a derived condition. In most panarthropod embryos, the embryonic midgut is already made of multiple cells , as is also observed in D. melanogaster . In P. caudatus embryos, the internal portion of the gut consists of 16 cells defining a tube and organized in three groups of four cells each and two posterior pairs of cells (Additional file 2: Video S1), a situation strikingly similar to the one described in C. elegans. However, successive rounds of molting seem to involve a general increase in the number of cells within the larval tissues and organs of P. caudatus (Figures 2 and 3), until reaching the polycytose situation of the intestine of adult priapulids. Notably, the priapulid hatching larva is non-feeding, as it lacks an oral and anal cuticular opening , and thus the olygocytose condition of the early post-embryonic intestine might be an adaptation to hatching with a yolk-rich immature gut. Taking everything into account, the development of a polycytose gut already during embryogenesis seems to be the ancestral condition in the Ecdysozoa.
Mesoderm in P. caudatus and its relationship to endoderm development
The endoderm often develops in close association with the mesoderm - the internal germ layer that generates the musculature, blood system, excretory organs, and skeleton - and thus the endoderm and mesoderm frequently influence each other’s subsequent development [4,5]. In line with the variability in endoderm development observed in the Ecdysozoa, mesoderm segregation and differentiation also show great diversity [7,38]. In the nematode C. elegans, most larval mesoderm originates from the MS cell in the eight-cell stage embryo (Figure 6A), which is the sister cell of the endodermal E cell, both coming from the mother EMS cell in the four-cell stage embryo . Ablation and cell culture studies have demonstrated that the E cell and its descendants have intrinsic properties to form polarized gut-like cells , and to pattern along the anteroposterior axis in a lineage-autonomous manner , although external factors and interactions with adjacent tissues, such as MS daughter cells  and the pharynx , are required for the proper definitive morphology of the digestive system. In early branching nematodes, there is no specification of the MS cell [53-55], and the formation of the embryonic midgut in relation to adjacent tissues has not been addressed. In the Nematomorpha, the exact origin of the mesoderm is not clear, although it appears as two lateral bands during gastrulation, surrounding the endoderm . In the arthropod D. melanogaster, the mesoderm forms in the ventral region of the embryo, and is separated from the anterior and posterior midgut primordia by the foregut and hindgut ectoderm, respectively [26,60] (Figure 6B). The ingression of the mesoderm creates a ventral furrow, and its differentiation into the visceral mesoderm is essential for the proper development of the midgut cells and the formation of a through gut . This situation seems to be common to most winged insects  and some apterygote (wingless) insects . In other yolk-rich panarthropod embryos, mesoderm development is more variable  and can occur from a small posteroventral area of the blastoderm (for example, onychophorans [65,76]), or from individual cells delaminating from the blastoderm (for example, in some myriapods ). By contrast, in those marine crustaceans with holoblastic cleavage and hollow blastulae, the mesoderm originates from a small subset of vegetal blastomeres internalized with the endoderm during gastrulation, usually in the form of two lateral bands [10,69]. Finally, in the tardigrade T. stephaniae, the mesoderm originates from a variable number of blastomeres that internalize and proliferate as two bands along the left and right sides of the embryo, giving rise to the somites .
The expression of the mesodermal gene twi in P. caudatus at the gastrula stage (Figure 3) indicates that mesoderm originates from the most vegetal/posterior endomesodermal cells of the gastrula, and extends anteriorly as two lateral rows. According to a previous study , these two lateral mesodermal rows form through active proliferation, rather than by continuous ingression of cells through the blastopore. No visceral musculature is formed during embryonic development (Figures 2 and 3), although the presence of visceral mesodermal precursors within the population of foxA-positive gut cells remains a possibility. The visceral musculature thus probably appears in subsequent larval stages, given that this tissue is present in adult priapulids. However, the internal portion of the gut develops in close contact with the forming body wall musculature, and thus reciprocal interactions between endoderm and mesoderm cannot be completely excluded. Considering the different mechanisms observed in ecdysozoans, the ancestral mode of mesoderm formation is likely by the specification and internalization of mesodermal precursors along with the endodermal cells at the vegetal pole, and the formation of two lateral mesodermal bands through active proliferation that enclose the developing endoderm. Further functional investigations in P. caudatus and other ecdysozoan groups will be required to understand if the similarities in the interactions between the mesoderm and the endoderm observed in D. melanogaster and vertebrate embryos represent cases of convergence, or instead reflect ancestral developmental mechanisms.
The question about the position of the mouth in the evolution of the Ecdysozoa
Conserved molecular patterning of the P. caudatus gut
As discussed above, the ontogeny and adult architecture of the digestive system is highly variable between ecdysozoan lineages. This observation raises questions regarding the extent of differences in the molecular mechanisms underpinning gut development, and how these changes account for the manifest diversity of gut architectures. In the nematode C. elegans, endoderm specification is triggered by the maternally supplied bZIP/homeodomain gene skn-1 (related to the nrf2 gene of vertebrates, and the cap’n’collar gene of D. melanogaster), which is required for proper specification of the ventral EMS cell . After the division of this cell, skn-1 activates a cascade of redundant pairs of GATA factors (med-1, med-2, end-1, end-3, elt-2, elt-4, and elt-7) that will lead to the establishment of endodermal fate in the E cell , but not in the MS cell . The Wnt pathway is also involved in this process , although this seems to be related to its general role in segregating cell fates along the anteroposterior axis . Additionally, the transcription factor pha-4, an ortholog of the endodermal marker foxA, is expressed throughout the pharynx and midgut [91-93], and orthologs of NK2.1, otx, FGF8/17/18, and foxQ2 (C25A1.2) are expressed and/or involved in pharynx development [19,46,94-96]. In nematodes, the nuclear hormone receptor family, and in particular the endodermal-related HNF4, has undergone extreme duplication , and many of the paralogs are expressed in different regions of the digestive system . The posterior region and the hindgut, which consists of eight cells derived from the ABp blastomere , also show expression of wnt1, evx, T-box genes (not bra, which seems to be absent in C. elegans), cdx, and pha-4 (foxA), among others [93,99-101] (see Figure 6F for a summary of expression data). In D. melanogaster, the specification of the midgut primordia is controlled by the terminal gap-gene huckebein (hkb), which controls endoderm specification at the amnioproctodeal invagination (posterior midgut), invagination of the anterior midgut, and specification of mesodermal precursors at the ventral furrow . In D. melanogaster, hkb is a core component of the terminal patterning system, a development pathway involved in setting up the anterior and posterior ends of the embryo in hexapod arthropods [103,104]. However, its enrolment in this developmental pathway seems to be an evolutionary novelty, probably unique to D. melanogaster and closely related species, and its ancestral function was likely related to the nervous system . The transcription factor forkhead (foxA); the GATA genes serpent, grain, and dGATAe (orthologs of the GATA456 subfamily); and the nuclear hormone receptor HNF4 are subsequently required for proper midgut development in D. melanogaster [105-107]. Additionally, other genes such as NK2.1, gsc, otx, foxQ2, and FGF8/17/18 are involved in the patterning of the head and foregut [47,49,108-110], and the genes bra, cdx, wnt1, evx, FGF8/17/18, and also foxA are required for the proper formation and patterning of the posterior region of the embryo [106,111-114] (Figure 6G). In the priapulid P. caudatus, the expression patterns of most of these genes exhibit significant similarities to the expression domains reported for C. elegans and D. melanogaster (Figure 6H). The F-box containing protein foxA is expressed in the foregut and inner gut , while a single GATA456 gene and the HNF4 ortholog are expressed in the anterior region of the internal alimentary canal. Together with gsc and otx , NK2.1, foxQ2, and FGF8/17/18 are expressed in the foregut, while wnt1, evx, and also FGF8/17/18 are detected in the ectodermal anus, as well as bra and cdx . The expression of the endodermal midgut markers GATA456 and HNF4 is likely limited to the three most anterior tetrads of the internal gut, and the observation of the hindgut genes foxA, bra, and cdx  in the region corresponding to the two most posterior duplets could indicate that these regions correspond to the endodermal midgut and internal ectodermal hindgut of the priapulid embryo, respectively. More detailed cell lineage analyses will be required to confirm this hypothesis. Although functional data are still lacking in P. caudatus, the comparison of expression data with that of the nematode C. elegans and the insect D. melanogaster reveals important similarities between these lineages of ecdysozoans (Figure 6F-H), mostly during the stages in which the gut is patterned into the three main regions. Notably, the overall patterning of the digestive system appears to be more conserved between P. caudatus and D. melanogaster, although P. caudatus and C. elegans would be considered morphologically more similar [115,116]. C. elegans differs mostly by the absence (for example, gsc and bra) or expansion (GATA456, HNF4) of some of the studied genes, which might be related to its high rate of genome evolution . Similarly, the differences in the earliest steps of endoderm development between C. elegans and D. melanogaster are probably due to their idiosyncratic early embryogenesis, as has also been shown in other bilaterian animals , and thus further work is needed to address the ancestral mechanism of endoderm specification for the Ecdysozoa. Nevertheless, our data on P. caudatus support the existence of a conserved molecular patterning program for the digestive system in the Ecdysozoa, despite the great differences in developmental modes and gut architectures.
The expression patterns of the above investigated genes in representative members of the ecdysozoan out-groups Spiralia (for example, the annelid Capitella teleta; Figure 6I) and Deuterostomia (for example, Branchiostoma floridae; Figure 6J) demonstrate that a similar system is also involved in gut regionalization outside the Ecdysozoa [48,51,52,118-128], although, in these organisms, the expression domains of particular genes often occur in, and extend to, different regions and germ layers. This observation, together with the similarities observed between P. caudatus, C. elegans, and D. melanogaster, strengthens the hypothesis of an ancestral molecular gut patterning system that is shared to a great extent between all the Ecdysozoa, despite morphological and developmental deviations being present in particular groups. Importantly, the molecular machinery that underlies early gut development in animals is much more similar than the developmental modes they undertake and the adult gut architectures they display (Figure 6). Therefore, the study of this common developmental toolkit alone cannot explain the vast morphological diversity of digestive tracts in animals. Differences in expression domains indicate that gene interactions and regulatory networks are probably variable, influenced by distinct developmental modes, early molecular/maternal inputs, and, most importantly, downstream effectors. Ultimately, the diversity of gut architectures also relies on molecular differences at more advanced stages of development. For instance, GATA factors activate effector genes required for intestinal cell differentiation in C. elegans [88,129,130], while triggering the epithelial-to-mesenchymal transition of the midgut primordia in the fly . In a more general context, our study shows that the investigation of general patterning mechanisms between animals cannot lead to the prediction of a morphological outcome. A deeper understanding of the vast morphological diversity of animal forms can thus only be gained by broader taxon sampling and the consideration in developmental studies of the more terminal ontogenetic events that are ultimately responsible for the final morphological outcomes.
Our comparative study of the development of P. caudatus, a representative of the sister group to all remaining ecdysozoans, shows that there are some primary features in the development of the digestive system that are likely to be ancestral for the Ecdysozoa, namely the formation of the endodermal midgut region from a single population of vegetal cells internalized during gastrulation, the ventral opening of the mouth and its subsequent shift to an anterior terminal position, and the development of the anus from the blastopore. Over evolutionary time, these characters have undergone great diversification and adaptation, as exemplified by the modes of gut development present in the two textbook invertebrate models, the nematode C. elegans and the fruit fly D. melanogaster. However, these extreme developmental divergences do not seem to be associated with a similar extent of molecular innovation in upstream patterning systems, as common transcriptional expression profiles are observed during the early stages of gut assembly among different ecdysozoan lineages. Our data not only shed light on the unexplored embryogenesis of the Priapulida and the evolution of the Ecdysozoa, but, importantly, also improve our understanding of the evolutionary changes that occurred in the lineages leading to C. elegans and D. melanogaster.
Animal collection, fertilization, and embryo fixation
Adult gravid specimens of P. caudatus were collected from Gullmarsfjorden (Fiskebäckskil, Sweden) in November in 2011, 2012 and 2013. Ovaries and testes were dissected, and kept in filtered deep seawater (FDSW). Oocytes were released by shaking the ovaries, and were fertilized with active diluted sperm from several males. Fertilized eggs were kept in petri dishes with FDSW at a constant temperature of 9°C, and washed daily with fresh FDSW to avoid bacterial and protozoan contamination. Embryos hatched 9 days after fertilization, and hatching larvae molted to the first lorica larvae 1 week thereafter, without any added food source. Before fixation, embryos were permeabilized with 0.05% thioglycolate, 0.01% pronase in FDSW for 45 min at 9°C. After three washes in FDSW, embryos were fixed in 4% paraformaldehyde in FDSW for 1 h at room temperature, followed by three washes in phosphate-buffered saline (PBS) with 0.1% Tween-20 (PTw). Hatching larvae and first lorica larvae were relaxed in 0.1% tricaine in FDSW for 30 s and fixed immediately in 4% paraformaldehyde in FDSW for 1 h at room temperature. Embryos and larvae fixed for immunohistochemical studies were stored in 0.1% sodium azide in PTw at 4°C. Samples fixed for gene expression studies were dehydrated in 50% methanol in PTw, washed once in 100% methanol, and stored in methanol at −20°C.
Cell proliferation was observed by the incorporation of the thymidine analog EdU during DNA replication. Batches of embryos at days 3.5 (n = 18), 4.5 (n = 16), and 5.5 (n = 19) of development were incubated for 3 h in FDSW supplemented with 10 μM EdU. After this pulse, the medium was changed several times to remove any traces of EdU. Treated embryos were permeabilized and fixed as described above, 6 h, 12 h, and 24 h after the start of the EdU pulse, and stored in 0.1% sodium azide in PTw at 4°C. Fluorescent labeling of the incorporated EdU was performed as recommended by the Click-it EdU Alexa Fluor 488 imaging kit (Life Technologies, NY, USA), and nuclei were counterstained with 0.01 mg/mL propidium iodide.
Embryos fixed and stored for immunohistochemical studies were washed several times in PBS before staining. Actin filaments and nuclei were labeled with 5 U/mL of Bodipy-FL phallacidin (Life Technologies, NY, USA) and 0.01 mg/mL propidium iodide (Sigma-Aldrich Chemie Gmbh Munich, Germany) in PBT (PBS, 0.2% TritonX-100, 0.1% bovine serum albumin) for 1 h at room temperature. Thereafter, embryos were washed in PBS for 1 h, dehydrated in a graded isopropanol series (70%, 85%, 95% in PBS, and twice in 100% for 30 to 60 s each) and cleared in Murray’s reagent (benzyl benzoate to benzyl alcohol, 2:1, v:v).
Gene expression studies
A fragment of NK2.1, and the full-length sequences of foxQ2, FGF8/17/18, GATA456, HNF4, wnt1, evx, and twi [GenBank: KP013750–KP013757] were identified from RNAseq data. Protein alignments were constructed with MAFFT , and poorly aligned regions were removed with Gblocks . RAxML  was used to infer gene orthologies (Additional file 5: Figure S4). Resulting trees were formatted with FigTree. Single colorimetric in situ hybridization was performed as described in . Fluorescent in situ hybridization of foxA in EdU-treated embryos was performed following the regular colorimetric protocol up to antibody incubation, when samples were incubated overnight with an anti-DIG POD-conjugated antibody (Roche, Indianapolis, IN, USA) diluted 1:250 in blocking solution. After extensive washes, the signal was developed with a TSA-Cy3 kit (Perkin-Elmer, Waltham, MA, USA) following manufacturer’s recommendations. The TSA reaction was stopped in detergent solution (1% Triton X-100, 1% SDS, 0.5% sodium deoxycholate, 50 mM Tris pH 8, 150 mM NaCl) at 60°C, and embryos washed several times in PTw afterwards. Subsequent fluorescent labeling of the EdU incorporation in these embryos was performed as suggested by the EdU kit manufacturer (Life Technologies).
Fluorescence-stained embryos and larvae cleared in Murray’s reagent were scanned with a Leica SP5 confocal laser scanning microscope (Leica, Wetzlar, Germany). Embryos exhibiting representative expression patterns of the analyzed genes were cleared in 70% glycerol in PTw, and imaged with a Zeiss Axiocam HRc connected to a Zeiss Axioscope Ax10 using bright field Nomarski optics (Zeiss, Oberkochen, Germany). Images were analyzed in Fiji and Photoshop CS6 (Adobe), and figure plates made with Illustrator CS6 (Adobe).
We thank the members of the Hejnol laboratory for support and discussions, Gemma S. Richards for a critical read of the manuscript, the staff at the Sven Lovén Centre for Marine Sciences for helping with the collections, and the two anonymous reviewers for their helpful comments. The study was funded by the core budget of the Sars Centre, and the collection trips were funded by the European Union Infrastructures Program (ASSEMBLE grant agreement no. 227799). JMMD is supported by Marie Curie IEF 329024 fellowship.
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