Fig. 5
From: Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors
Cell cycle dependent dynamics of signaling in the mating pathway. a, b Yeast cells expressing the Ste7DS-NLS-RFP sensor and Whi5 tagged with mCitrine (a) or Yox-1 tagged with mCitrine (b) imaged before and after stimulation with α-factor. c, d Correlation between the measured Whi5 (Nc = 616, c) and Yox1 (Nc = 698, d) nuclear enrichment (nuclear – cytoplasmic intensities) measured before stimulation and the sensor’s final response. The scatter plot is split in four quadrants. The blue dots represent cells with an enriched nuclear marker, while the green dots represent cells where no enrichment is detected. The dark and light coloured dots represent, respectively, responding and non-responding cells. e, f Average response of the four sub-populations of cells in the four quadrants delimited on panels c (Whi5, e) and d (Yox1, f). The colour code is the same as in (c) and (d)