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Fig. 7 | BMC Biology

Fig. 7

From: Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors

Fig. 7

Mpk1 activity dynamics upon zymolyase or hypo-osmotic stresses. a Schematic of the Mpk1 sensor and alignment of the docking sites present in Mkk1 and Mkk2. b Scheme of the activation of Mpk1 by zymolyase or hypo-osmotic stress. c Dynamic localization of the Mkk2DS1-100-NLS-RFP sensor quantified by its change in nuclear-to-cytoplasmic ratio upon stimulation by zymolyase 3 U/mL at time 0 in WT (red, Nc = 313), ste11∆ (blue, Nc = 160), and bck1∆ (green, Nc = 80). d Histograms of the final response after zymolyase stress measured in the WT and two deletion strains. e Mpk1 sensor relocation upon hypo-osmotic shock performed in microfluidic devices where the sorbitol concentration in the medium is lowered from 1 M to 0.5 M: WT (red, Nc = 168), ste11∆ (blue, Nc = 180), and bck1∆ (green, Nc = 217). f Histograms of the final response after hypo-osmotic stress measured in the WT and two deletion strains. In panels D and F the asterisks designate distributions that are significantly different (t-test, P <0.001) from the bck1∆ control

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