Fig. 6
Histograms show dCTCF or CP190 occupancy in chromatin isolated from mid-late pupa at sequences containing the BX-C insulators Fab-3, Fab-4, Mcp, Fab-6, Fab-8, the Abd-D promoter, and several previously defined dCTCF insulators (9A1, 21E2, 24C4, 27B2 and 57B4R). Cross-linked chromatin prepared from wild-type (WT) (y 1 w 1118) pupae and homozygous GE24185 (GE/GE) mutant pupae was immunoprecipitated with antibodies directed against the N-terminal domain of dCTCF and CP190. Sequences from tub, rpl32, and 62D regions were used as negative controls for dCTCF and CP190 association. 62D is an example of a sequence in which CP190 occupancy is independent of dCTCF. The left axis shows the scale for dCTCF enrichment, while the right axis shows the scale for CP190 enrichment. Each chromatin immunoprecipitation experiment was performed in at least two independent biological replicates. Error bars show standard deviations of quadruplicate PCR measurements. The results are presented as a percentage of input DNA