Table 4 Composition of protein bound and free intracellular amino acids of P. pastoris grown on glucose or methanol/glycerol in chemostats at μ = 0.1 h⁻¹

Asx

48.8

30.2

0.69

5.42

2.91

0.89

0.00

Ala

32.1

20.2

0.67

0.90

1.02

−0.18

0.28

Arg

29.6

25.9

0.19

11.1

12.1

−0.12

0.47

Cys

5.59

4.56

0.30

–

–

–

–

Glx

87.0

68.0

0.36

22.3

29.4

−0.40

0.00

Gly

18.7

11.5

0.70

–

–

–

–

His

11.9

7.33

0.70

0.76

0.74

0.03

0.78

Ile

17.8

10.6

0.76

0.05

0.04

0.28

0.00

Leu

36.0

22.6

0.67

0.10

0.08

0.35

0.00

Lys

37.5

24.5

0.62

0.76

0.92

−0.28

0.18

Met

5.34

4.33

0.30

0.04

1.08

−4.63

0.00

Phe

20.0

12.9

0.63

0.03

0.03

0.03

0.56

Pro

–

–

–

0.92

2.76

−1.58

0.00

Ser

25.9

19.0

0.44

0.25

0.52

−1.04

0.00

Thr

28.2

19.3

0.55

0.24

0.27

−0.14

0.42

Tyr

17.3

8.89

0.96

0.06

0.05

0.18

0.02

Val

26.4

15.7

0.75

0.25

0.14

0.83

0.00

Total amino acids [mg/g Cell dry weight (CDW)]

448

306

0.55

43.2

52.1

−0.27

0.03

Total protein content [mg/gCDW]

540

390

0.47

–

–

–

–