Fig. 4

Effect of DMNT on Ca²⁺ responses in female ALs to the synthetic volatile mixtures. a Computed map showing the areas of ordinary glomeruli from which responses were calculated (left) and representative false colour-coded images of Ca²⁺ responses to Mix-5 (concentrations 1 and 2) and DMNT (0.1–10 μg) (right). Images were collectively scaled to strongest activation. b Ca²⁺ responses over all glomeruli (n = 81) from 13 moths to Mix-5 concentration 1 (open triangles), 2 (solid squares), mixed with DMNT (0.1 to 10 μg), and DMNT alone (open circles). Symbols represent the mean values of maximum Ca²⁺ response (+SE). c Ca²⁺ responses of individual ordinary glomeruli (n = 13) to Mix-5 concentration 1 (light grey), 2 (dark grey) mixed with DMNT, and DMNT alone (white). Squares represent the mean values of maximum relative Ca²⁺ response (+SE). Different letters denote significantly different responses (lme, P < 0.05) within treatments (groups of colour)