Fig. 5

Random forest analysis of seminal fluid composition under variable sperm competition risk. The figure plots the seven secreted seminal vesicle proteins important for classifying samples according to the sperm competition risk treatment, based on variable importance scores. Standardised abundance scores of each protein are shown (blue symbols: high sperm competition (HSC); red symbols: low sperm competition (LSC); mean ± 95 % confidence intervals). Proteins are ordered according to their variable importance score as per Table 3 (see Table 3 for UniProt IDs). Analysis is based on data requiring at least three unique peptides for quantification and identification, with normalisation to ejaculated proteins (see Methods)