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Fig. 5 | BMC Biology

Fig. 5

From: Loss of Ezh2 promotes a midbrain-to-forebrain identity switch by direct gene derepression and Wnt-dependent regulation

Fig. 5

Ezh2 regulates midbrain identity indirectly. (a) qRT-PCR for Ezh2, Pax3, and Pax7 on midbrain tissue isolated at E11.5 and E12.5 reveals a downregulation of midbrain transcription factors Pax3 and Pax7 in the absence of Ezh2 after E11.5. n ≥3 in each group, ***P ≤0.001, Student’s t-test. (b, c) Immunostaining for Pax3 (b) and Pax7 (c) on sagittal midbrain sections shows diminished protein levels at E12.5 (magnification in lower panels, asterisk defines basal). The cartoon insets indicate regular Pax3 and Pax7 expression at E12.5, respectively. DAPI staining serves as nuclear marker: b, c; Scale bars: b, c, 500 μm (upper panel); b, c, 40 μm (lower panel); Error bars indicate SD; MB, Midbrain

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