Fig. 2
From: iCLIP identifies novel roles for SAFB1 in regulating RNA processing and neuronal function
SAFB1 knockdown mediates changes in the expression of specific RNA isoforms. RT-PCR analysis of genes with crosslinks identified by iCLIP was performed in such a way as to examine the possibility of transcript-specific alterations. a RNA was isolated from SHSY5Y cells following siRNA mediated SAFB1 knockdown and SAFB1 and SAFB2 RNA levels measured. b Specific primer sets were designed and used to measure the expression of NCAM1, SYNPO2, ASTN2 and PDE4B isoforms. The results were obtained following three independent experiments. Statistical analysis was by ANOVA followed by post-hoc t-tests. *P <0.05, **P <0.01