Fig. 7
From: iCLIP identifies novel roles for SAFB1 in regulating RNA processing and neuronal function
SAFB1 regulated dendritic spine size in primary hippocampal neurons. a Immunocytochemical analyses show SAFB1 is highly expressed in the hippocampus (i). SAFB1 is clearly evident in pyramidal neuron cell bodies in the CA1 region of the hippocampus ((i) red SAFB1 immunofluorescence) and a neurofilament stain of dendrites (green immunofluorescence (ii) second panel) shows SAFB1 is located exclusively in neuronal cell bodies. Primary hippocampal neurons were transduced with SAFB-EGFP or EGFP alone (confocal images shown in (b) and confocal microscopy used to measure spine (c) area, (d) size, and (e) density. Spine size measures were analysed by cumulative frequency using the Kolmogorov-Smirnov test (b, d = 0.1075; P <0.001) and by t-test (c) ***P <0.001. Values shown in bar graphs are means ± SD