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Fig. 2 | BMC Biology

Fig. 2

From: Tankyrase inhibition impairs directional migration and invasion of lung cancer cells by affecting microtubule dynamics and polarity signals

Fig. 2

Tankyrase 1 and 2 (TNKS/2) inhibition by XAV939 or JNJ-BJ impairs hepatocyte growth factor (HGF)-dependent cell migration and invasion in non-small cell lug cancer (NSCLC) cells. a Western blot showing the amount of axin1 in total protein extracts from A549 cells treated with a range of concentrations of XAV939 or JNJ-BJ for 24 h. Actin was used as a loading control. Molecular weights are indicated. b Dose-dependent inhibition of HGF-induced invasion in A549 cells by treatment with increasing concentrations of XAV939 or JNJ-BJ (HGF, 50 ng/mL). Quantification was carried out by ImageJ software. TNKSi TNKS/2 inhibitor. Data are the means (lines) ± standard error of the mean (SEM) of three independent experiments (diamonds), each performed in technical duplicate. c Western blot analysis showing the levels of axin1 in whole-cell extracts from the indicated NSCLC cell lines. Before protein extraction, cells were incubated with 10 μM XAV939 or JNJ-BJ for 24 h. Actin was used as a loading control. Molecular weights are indicated. d ImageJ quantification of HGF-induced invasion in the indicated cell lines upon TNKS/2 blockade (HGF, 50 ng/mL; TNKS/2 inhibitors, 10 μM). Data are the means (lines) ± SEM of three or four independent experiments (diamonds), each performed in technical duplicate. e Representative images of a scratch assay performed with the indicated cell lines in the following experimental conditions (from left to right): non-stimulated control wound (Day 0); HGF-treated cells 24 h after wounding (HGF, 50 ng/mL); HGF-treated and XAV939/JNJ-BJ-treated cells 24 h after wounding (HGF, 50 ng/mL; TNKS/2 inhibitors, 10 μM). Plots on the right depict ImageJ quantification of wound repair inhibition 24 h after scratch. Data are the means (lines) ± SEM of three independent experiments (diamonds), each performed in technical duplicate or triplicate. *P < 0.05; **P < 0.01; ***P < 0.001 by two-tailed Student’s t-test (TNKSi-treated versus untreated [dimethyl sulfoxide, DMSO]). Raw data for panels b, d, and e are shown in Additional file 3

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