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Fig. 5 | BMC Biology

Fig. 5

From: Tankyrase inhibition impairs directional migration and invasion of lung cancer cells by affecting microtubule dynamics and polarity signals

Fig. 5

Centrosome reorientation is retarded by tankyrase I and 2 (TNKS/2) inhibitors in migrating cells. Representative confocal images of centrosome localization in migrating A549 cells. Wound-edge A549 cells, with or without TNKS/2 inhibitors (10 μM), were stimulated with hepatocyte growth factor (HGF) (50 ng/mL) for 4 h. Immunofluorescence staining of centrosomes was carried out using anti-γ-tubulin antibody. Centrosome polarization was assessed at the indicated time points. Pie charts display the percentage distribution of distinct angles (α) formed by the nuclear-centrosome axis (red arrows) and a line perpendicular to the wound (white arrows). Centrosomes were considered oriented when α < 30°. A minimum of 136 cells was analyzed for centrosome polarization in three independent experiments. P-values were calculated by the Chi-square test using Graphpad Quickcalcs software. Scale bar, 7 μm. Raw data are shown in Additional file 15. DMSO dimethyl sulfoxide

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