Fig. 4

Expression of other Wnt genes and AP axis markers during E. multilocularis metacestode development. Whole-mount in situ hybridization signal is shown in green and nuclear DAPI staining in blue. PS Act, Activated protoscoleces; PS st 1 and 2: First and second stage of protoscolex development following the system of Leducq and Gabrion [25]; GL: Germinal layer; BC bud: Early brood capsule bud (shown from above for all genes except for em-wnt11A and em-wnt1, for which it is shown from a side); rb, Rostellar bulb; rpb, Rostellar pre-bulb; s, Suckers; r, Rostellum. Bars: 20 μm