Fig. 4

Olfr692-positive cells co-express TrpC2 and are located in the basal layer of the vomeronasal organ. (a, b) VNO sections subjected to double fluorescent in situ hybridization (ISH) showing co-expression of Olfr692 (red) and TrpC2 (green), coding for an ion channel characteristically expressed in vomeronasal neurons (higher magnification image in b). (c, d) Double fluorescent ISH to evaluate expression of genes for G protein subunits Gαo (c), a basal VNO zone marker, or Gαi2 (d), an apical zone marker, in Olfr692-expressing VNO cells. Number of imaged sections and quantification of co-labeling counts are summarized in Additional file 10: Table S2. lu, VNO lumen. Scale bars represent 100 μm and are the same as in the left top panel, except in b. Nuclear To-Pro-3 labeling is light red in d and blue elsewhere