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Fig. 5 | BMC Biology

Fig. 5

From: Mechanosensory organ regeneration in zebrafish depends on a population of multipotent progenitor cells kept latent by Schwann cells

Fig. 5

Neuromast regeneration success is inversely correlated with the size of the interneuromastic cells (INC) gap generated by electroablation. The L3 neuromast of tg(cxcr4b:mCherry;foxd3:GFP) larvae was electroablated. At 2 hours post injury (hpi), we individually injured larvae and measured the length of the gap between remaining INCs (labeled in red) and Schwann cells (SCs, labeled in green). At 72 hpi, we scored the regeneration of the L3 neuromast and compared the two outcomes (regeneration or no regeneration) after measuring the average gap size in each group. As is shown in a, larvae that could not regenerate the L3 neuromast presented a larger gap between INCs compared to those that regenerated (n = 64). We did the same comparison examining the size of the SC gap and found no effect in this case (b; n = 64). Calculating the ratio between INC and SC gap size again produced a significant difference when regenerating versus non-regenerating outcomes were compared (c; n = 64). Further, there was no difference in the ratio of the INC/SC gap between larvae that regenerated at 48 hpi versus those that regenerated at 72 hpi (d; n = 27). * p < 0.05; n.s. not significant. Further details on replicates are provided in “Quantifications and statistical analysis” in the “Methods” section

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