Fig. 4

aPKC is recycled via Nuf-RE. a-b Confocal images of wing discs containing sec6 (a) or sec5 (b) clones. In the absence of sec6 (a) aPKC accumulates in the mutant cells (red, right panel). In sec5 clones (b) aPKC (blue, right panel) and Nuf (red, middle panel) accumulate. c When Rab11 is silenced, aPKC (red) is disrupted from the apico-lateral cortex (arrows) accumulating in the cytoplasm (right). d Wing disc stained with aPKC (green) and the early recycling endosome marker Rab5 (red). Arrows point to co-stained endosomes. e-f aPKC (red) is reduced in nuf ^KG00314 (e) or nuf ¹ (f) mutant clones. g-h Fluorescence levels of aPKC comparing wild-type (red) with clone cells (blue) for nuf ^KG00314 (g, left) or nuf ¹ (h, right). Clones are marked by the absence of GFP (a and b) or β-gal (e-f) or expression of GFP (c) in green. (a-c, e-f) Lower panels show sagittal views of the clones and are marked by arrows. aPKC atypical PKC, RE recycling endosome, GFP green fluorescent protein